Chrysanthemum flowers Chrysanthemum x grandiflorum (Ramat.) Kitam., are commercially significant worldwide as there are large number of cultivars for cut flowers, pot flowers, and garden flowers. Commercial in vitro multiplication of chrysanthemum is often based on stem nodal explants with lateral meristems. This chapter describes a protocol for in vitro propagation from stem nodal explants and by direct organogenesis from pedicel explants producing large number of true-to-type plantlets in 4–8 week on Murashige and Skoog (MS) based media. Also, true mutants with changed flower color are obtained without producing chimeras after gamma-irradiation in mutation breeding.