Analyzing Maize Meiotic Chromosomes with Super-Resolution Structured Illumination Microscopy
The success of meiosis depends on intricate coordination of a series of unique cellular processes to ensure proper chromosome segregation. Many proteins involved in these cellular events are directly or indirectly associated with chromosomes, especially those required for homologous recombination. These meiotic processes have been explored extensively by conventional light microscopy. However, many features of interest, such as chromatin organization, recombination nodules, or the synaptonemal complex are beyond the resolution of conventional wide-field microscopy. Moreover, in most sample preparation techniques for light microscopy, meiotic cells are squashed, which destroys the spatial organization of the nucleus. Here, I describe a protocol to analyze maize meiotic chromosomes by three-dimensional structured illumination microscopy (3D-SIM), a recently developed high-resolution microscopy technique. This protocol can be used to examine protein localizations at a high resolution level by immunofluorescence.
- 黄栌(Cotinus coggygria var.pubescens)
- 苘麻
- 番仔林投
- Chromatin Immunoprecipitation to Identify Global Targets of WRKY Transcription Factor Family Members Involved in Plant Immunity
- 油棕
- Isolation of Chloroplast Proteins from Arabidopsis thaliana for Proteome Analysis
- 苜蓿(Medicago sativa)
- Insect Maintenance and Transmission
- Isotopomer Measurement Techniques in Metabolic Flux Analysis II: Mass Spectrometry
- Detection of mRNA Expression Patterns by Nonradioactive In Situ Hybridization on Histological Sections of Floral Tissue