Phaseolus Immature Embryo Rescue Technology
Predominant among the production constraints of the common bean Phaseolus vulgaris are infestation of Ascochyta blight, Bean Golden Mosaic virus (BGMV), and Bean Fly. Interbreeding with Phaseolus �coccineus L. and/or Phaseolus polyanthus Greenm has been shown to provide P. vulgaris with greater resistance to these diseases. For interspecific crosses to be successful, it is important to use P. coccineus and P. polyanthus as female parents; this prevents rapid reversal to the recurrent parent P. vulgaris . Although incompatibility barriers are post-zygotic, early hybrid embryo abortion limits the success of F1 crosses. While rescue techniques for globular and early heart-shaped embryos have improved in recent years, �success in hybridization remains very low. In this study, we describe six steps that allowed us to rescue 2-day-old P. vulgaris embryos using a pod culture technique. Our methods consisted of (i) pod culture, (ii) extraction and culture of immature embryos, (iii) dehydration of embryos, (iv) germination of embryos, (v) rooting of developed shoots, and (vi) hardening of plantlets.
- 根系活力的测定[TTC法]
- 实验87 华氏呼吸计
- 谷精草科 (Eriocaulaceae)
- 桉属(Eucalyptus)
- Cell Lineage Analyses in Living Tissues
- Phosphorylation Studies Using Plant Protein Microarrays
- Quantitative Analysis of Protein Phosphorylation Using Two-Dimensional Difference Gel Electrophoresis
- Luciferases as Reporter Genes
- Deducing Intracellular Distributions of Metabolic Pathways from Genomic Data
- Electrofusion of Plant Cells