In Situ Detection of miRNAs Using LNA Probes
关键词: situ detection mirnas来源: 互联网
A spatial and temporal analysis of miRNA accumulation by in situ analyses is the prerequisite of understanding the precise biological functions of miRNAs. Since miRNAs are very short molecules, their in situ analysis is technically demanding. Here, we describe a protocol for miRNA in situ detection in plants based on LNA-modified oligonucleotides probes. LNA modification significantly enhances the sensitivity and specificity of miRNA detecting probes and provides relatively easy in situ miRNA detection.
推荐方法
- 植物体内可溶性蛋白质含量的测定:LoWry法(劳里法)
- 单性结实
- 蒸腾效率(transpiration efficiency)
- 壳斗科(Fagaceae)
- 杉松
- 枣(Zizyphus jujuba)
- Chloroplast Transformation
- An Array Platform for Identification of Stress-Responsive MicroRNAs in Plants
- Targeted Mutagenesis in Arabidopsis Using Zinc-Finger Nucleases
- Procedures for Plant Rhabdovirus Purification, Polyribosome Isolation, and Replicase Extraction