Preparation of Native and Recombinant Light-Harvesting Chlorophyll-a/b Complex
关键词: preparation native recombinant来源: 互联网
Procedures to isolate native light-harvesting chlorophyll-a/b complex (LHCIIb) and to reconstitute recombinant LHCIIb are described. Separation of trimeric from monomeric forms and free pigment by sucrose density-gradient ultracentrifugation can be applied to both native and reconstituted complexes. The preparations are characterized by their pigment composition, protein pattern and spectral properties.
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