Metabolic Engineering by Plastid Transformation as a Strategy to Modulate Isoprenoid Yield in Plants
Plants synthesize a large number of isoprenoid compounds that have diverse structures and functions. All isoprenoids are synthesized through consecutive condensation of five-carbon precursors, isopentenyl diphosphate (IPP) and its allyl isomer dimethylallyl diphosphate (DMAPP). With recent success in the cloning of genes that encode the enzymes of isoprenoid biosynthesis, genetic engineering strategies for the improvement of plant isoprenoid metabolism have emerged. Plastid transformation technology offers attractive features in plant genetic engineering. It has many advantages over nuclear genome transformation: high-level foreign protein expression, no need for a transit peptide, absence of gene silencing, and convenient transgene stacking in operons. We demonstrated that this technology is a remarkable tool for the production of isoprenoids in plants through metabolic engineering. The expression of bacterial genes encoding CrtW (β-carotene ketolase) and CrtZ (β-carotene hydroxylase) or cyanobacterial genes encoding DXR (1-deoxy-d -xylulose-5-phosphate reductoisomerase) in the plastid genome leads to alteration in isoprenoid content of tobacco leaves.
- 植物营养器官外部形态与内部结构的观察
- 厚壁组织
- 植物(plants)
- 小麦锈病
- 二叠纪植物群
- 苜蓿
- 植物地理学(botanical geography)
- Application of Yeast-Two Hybrid Assay to Chemical Genomic Screens: A High-Throughput System to Identify Novel Molecules Modulati
- Identification and Characterization of Quantitative Trait Loci that Control seed Dormancy in Arabidopsis
- Isolation of Nuclear Proteins