Transient Transformation of Plants
关键词: transient transformation plants来源: 互联网
Transient expression in plants is a valuable tool for many aspects of functional genomics and promoter testing. It can be used both to over-express and to silence candidate genes. It is also scaleable and provides a viable alternative to microbial fermentation and animal cell culture for the production of recombinant proteins. It does not depend on chromosomal integration of heterologous DNA so is a relatively facile procedure and can lead to high levels of transgene expression. Recombinant DNA can be introduced into plant cells via physical methods, via Agrobacterium or via viral vectors.
推荐方法
- 细菌的分离培养、纯培养和生化试验
- 渗透势(osmotic potential)
- 鹿角菜(Pelvetia siliquosa)
- 观音座莲目(Marattiales)
- 天竺葵(Pelargonium hortorum)
- 蓖麻
- 芦荟
- Screening for Transgenic Lines with Stable and Suitable Accumulation Levels of a Heterologous Protein
- Potato Transformation
- Appendix B: Plant Biotechnology and Tissue Culture Resources in the Internet