By insertion into an individual xylem vessel at the root base, the multifunctional xylem probe allows the monitoring of the xylem pressure, the radial electrical gradients in the root (the so-called trans-root potential, TRP), as well as the activity of a particular ion such as K+ in the xylem sap of intact, transpiring plants. The biophysical and physiological significance of these parameters with respect to salt stress is briefly explained, and the assembly of the probe, the setup used for these measurements, and the experimental procedure are outlined in detail.