ThiolDisulfide Redox Proteomics in Plant Research
Abiotic stresses often cause metabolic imbalances which affect cellular redox homeostasis and alter the rate of reduction state of functional and regulatory protein thiols and the rate of reactive oxygen species release. Excessive displacement from redox equilibrium causes oxidative damage to cell structures and may elicit cell death. The understanding of the cell response to progressive stress must include knowledge on the thiol redox state of specific proteins. This chapter describes selected gel-based biochemical methods (i) to identify thiol–disulfide redox proteins that undergo major redox-dependent conformational changes by 2D redox SDS-PAGE and (ii) to determine the thiol redox state of proteins by sequential blocking and labeling with N -ethylmaleimide and methoxypolyethylene glycol maleimide-5000 (mPEG-Mal-5000). Both sets of methods provide experimental information that defines the redox proteome of the cell.
- 百合目
- 桃
- 雄生殖托(antheridiophore)
- 假果
- Structural Proteins of the Primary Cell Wall: Extraction, Purification, and Analysis
- 紫苏
- 臭牡丹
- 本山
- High-Frequency and Efficient Agrobacterium-Mediated Transformation ofArabidopsis thaliana Ecotypes C24 and Landsberg erecta Usin
- Engineering the Plastid Genome of Nicotiana sylvestris, a Diploid Model Species for Plastid Genetics