In Vitro Propagation of Ash (Fraxinus excelsior L.) by Somatic Embryogenesis
Induction of somatic embryogenesis is described in common ash (Fraxinus excelsior L.). Embryogenic tissues are obtained from immature zygotic embryos and cultured on a modified Murashige and Skoog (MS) medium containing 8.8 μM 2,4-dichlorophenoxyacetic acid and 4.4 μM benzyl-adenine. Embryogenic tissue is subcultured and multiplied on medium supplemented with reduced concentration of plant growth hormones. Somatic embryos develop and mature by transfer to hormone-free medium and subsequent culture on medium containing low amount of benzyladenine. Somatic embryo germination and conversion are enhanced by cold storage at 4�C and successive transfer onto Woody Plant Medium (WPM). Fully developed plantlets are then transferred to pots and acclimatized in the greenhouse equipped with a mist system.
- 种子植物标本采集、制作与保存
- 人工种子(artificial seeds)
- 紫笋
- 摘心
- Maize Somatic Embryogenesis: Recent Features to Improve Plant Regeneration
- 使君子
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- Expression and Purification of Active Protein Kinases from Wheat Germ Extracts
- Low-Resolution Mapping of Untagged Mutations
- Elementary Flux Modes, Flux Balance Analysis, and Their Application to Plant Metabolism