Genetic Transformation Protocols Using Zygotic Embryos as Explants: An Overview
Genetic transformation of plants is an innovative research tool which has practical significance for the development of new and improved genotypes or cultivars. However, stable introduction of genes of interest into nuclear genomes depends on several factors such as the choice of target tissue, the method of DNA delivery in the target tissue, and the appropriate method to select the transformed plants. Mature or immature zygotic embryos have been a popular choice as explant or target tissue for genetic transformation in both angiosperms and gymnosperms. As a result, considerable protocols have emerged in the literature which have been optimized for various plant species in terms of transformation methods and selection procedures for transformed plants. This article summarizes the recent advances in plant transformation using zygotic embryos as explants.
- 叶绿体被膜完整度的测定
- 果实的类型
- 美人蕉叶柄中的薄壁组织
- 细胞壁(cell wall)
- 黄丝藻属 (Tribonema)
- 白兰花
- Reporter Gene-Based Recombination Lines for Studies of Genome Stability
- Sequential Replicas for In Vivo Imaging of Growing Organ Surfaces
- Agrobacterium-Mediated Transformation of Maize (Zea mays) Immature Embryos
- EMS Mutagenesis of Arabidopsis