Micropropagation of Vaccinium sp. by In Vitro Axillary Shoot Proliferation
关键词: micropropagation vaccinium来源: 互联网
The Vaccinium genus contains several valuable fruit and ornamental species, among others: highbush blueberry (Vaccinium � corymbosum L.), cranberry (Vaccinium macrocarpon Ait.), and lingonberry (Vaccinium vitis-idaea L.). In some most popular and valuable cultivars, the conventional propagation methods, exploiting hard or soft wood cuttings, are inefficient. The demand for nursery plants could be fulfilled only by micropropagation. In principle cultivars are propagated in vitro through similar three-stage method, based on subculture of shoot explants on different culture media supplemented with IAA (0–4 mg/L) and 2iP (5–10 mg/L), and rooting shoots in vivo. The obtained plantlets are transferred to peat substrate and grown in the glasshouse until the end of growing period. The development of adventitious shoots should be monitored and controlled during in vitro stages. Many clones have specific requirements for growing conditions and/or are recalcitrant.
推荐方法
- 希尔反应的观察
- 大叶藓属(Rhodobryum)
- 初生生长(primary growth)
- 紫色马勃
- 腰果(Anacardium occidentale)
- Jasmonic AcidAmino Acid Conjugation Enzyme Assays
- 周皮
- Bridging the Gene-to-Function Knowledge Gap Through Functional Genomics
- Vegetative Propagation of Cacti and Other Succulents In Vitro
- Reverse Genetics in Medicago truncatula Using Tnt1 Insertion Mutants