Development of an Efficient Inverse PCR Method for Isolating Gene Tags from T-DNA Insertional Mutants in Rice
The central goal of current genomics research in plants, as in other organisms, is to elucidate the functions of every gene. Insertional mutagenesis using known DNA sequences such as T-DNA is a powerful tool in functional genomics. Development of efficient methods for isolating the genomic sequences flanking insertion elements accelerates the systematic cataloging of insertional mutants, and thus allows functions to be assigned to uncharacterized genes via reverse genetic approaches. In our current study, we report a rapid and efficient inverse PCR (iPCR) method for the isolation of gene tags in T-DNA mutant lines of rice (Oryza sativa ), a model monocot plant.
- 红豆杉科(Taxaceae)
- 真菌
- 菱形藻属(Nitzschia)
- 根尖(root tip)
- 当归
- 桑(Morus alba)
- A Time Course for the Analysis of Meiotic Progression in Arabidopsis thaliana
- 石竹 Diranthus chinensis
- Quantifying the Amount and Activity of Rubisco in Leaves
- In Planta AgrobacteriumMediated Transformation of Adult Arabidopsis thaliana Plants by Vacuum Infiltration