In Situ Analysis of DNA Methylation in Plants
Epigenetic changes in the plant genome are associated with differential genome methylation, histone modifications, and the binding of various chromatin-binding factors. Methylation of cytosine residues is one of the most versatile mechanisms of epigenetic regulation. The analysis of DNA methylation can be performed in different ways. However, most of these procedures involve the extraction of chromatin from cells with further isolation and analysis of DNA. Modest success has been achieved in DNA methylation analysis in plant tissues in situ. Here, we present an in situ method for DNA methylation analysis, which has high sensitivity and good reproducibility.
- 石蜡切片操作
- 地衣(Lichens)
- 植物种群的进化和选择
- 茎卷须
- 甘蔗(Sarrcharym officinarum)
- 高粱蚜 (Melanaphis sacchari)
- QTL Analysis and Map-Based Cloning of Salt Tolerance Gene in Rice
- Profiling of Hormones and Related Metabolites in Seed Dormancy and Germination Studies
- Functional Complementation of Yeast Mutants to Study Plant Signalling Pathways
- Enzyme Extraction and Assessment of Enzyme Production