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Analytical Ultracentrifugation: Sedimentation Velocity Analysis

关键词： sedimentation velocity analysis来源：互联网

Abstract
Table of Contents
Literature Cited

Abstract

The analytical ultracentrifuge is a high speed centrifuge with an optical system allowing observation of the concentration of macromolecules as a function of radius and time. In sedimentation velocity experiments,relatively high speeds are used so that a boundary is formed between the solution of sedimenting macromolecule and the buffer in which it is dissolved. Analysis of the rate boundary movement and evolution of its shape can yield information about the molar masses of species present as well as stoichiometries and equilibrium constants for their interactions. This overview discusses crucial issues pertaining to sedimentation velocity experiments, including non?interacting and interacting systems, ideality and non?ideality, and reversible versus kinetically limited equilibrium scenarios.

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Sedimentation Velocity Theory
Experimental Design and Protocols
Data Analysis and Interpretation
Tables

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Literature Cited

Literature Cited
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	Stafford, W.F. 1994b. Sedimentation boundary analysis of interacting systems: Use of the apparent sedimentation coefficient distribution function. In Modern Analytical Ultracentrifugation: Acquisition and Interpretation of Data for Biological and Synthetic Polymer Systems (T.M. Schuster and T.M. Laueeds.) pp. 119‐137. Birkhäuser Boston Boston,Mass.
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	Williams, J.W., Van Holde, K.E., Baldwin, R.L., and Fujita, H. 1958. The theory of sedimentation analysis. Chem. Rev. 58:715‐806.
Key References
	Williams et al., 1958. See above.
	This selection of three pieces of older literature contains a tremendous amount of information that is very germane to modern day sedimentation analysis.
	Schachman, 1959. See above.
	Claesson and Moring‐Claesson, 1961. See above.

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