Unravelling the Ecology of Antibiotic Resistant Bacteria in the Nasopharynx
To study the dynamics and diversity of pneumococcal carriage and antibiotic resistance, a more thorough and systematic approach has been employed compared with routine surveillance of serotype and anti�biotic resistance. Up to ten pneumococcal isolates from pernasal (nose) and oropharyngeal (throat) sites are isolated and characterised. Our carriage studies have revealed a diverse community of pneumococci with multiple strains colonising the nasopharynx of children. In Tanzanian children less than 6 years of age, up to six serotypes and up to six different antibiotic sensitivities (as distinguished by at least a fourfold difference in the minimum inhibitory concentration) have been found. Serotyping by the Quelling reaction is prone to inaccuracy and requires expensive serological reagents. To improve the accuracy and reduce the costs, an alternative capsular typing DNA-based method has been developed. This chapter will describe the methods we have employed with emphasis on the capsular typing method.
- 现代免疫学时期
- 疫苗接种的无形价值
- 猪免疫球蛋白G3(IgG3)酶联免疫分析(ELISA)
- Solid Phase Assay Measuring C4d Deposition to Determine Complement Fixation by HLA-Specific Antibodies
- Quantitating MHC Class I Ligand Production and Presentation Using TCR-Like Antibodies
- Phage Display Technology
- Mouse Immune Libraries for the Generation of ScFv Fragments Directed Against Human Cell Surface Antigens
- Measurement of Eosinophil Accumulation In vivo
- Live Viral Vectors: Construction of a Replication-Deficient Recombinant Adenovirus
- Enrichment and Characterization of Glycopeptide Epitopes from Complex Mixtures