Selection of VHHs Under Application Conditions
The successful application of antibody fragments such as VHHs in diagnostic assays, affinity purification, imaging, or therapy is not determined by the specificity and affinity of the antibody fragment alone. The ability to bind the target protein in the environment in which the antibody fragment is intended to functionally perform determines to a great extent its success. To identify antibodies with the required stability profile selection of naturally occurring variants from an immune library or mutants from an engineered library should be performed via phage display. The conditions under which the designed antibodies displayed on phage bind to the target ideally should mimic the environment in which the antibody should be effective. After selection individual antibodies need to be tested in appropriate screening assays, again taking into account the conditions under which the antibody should bind to the target and induce the desired effect.
- 影响制备高效价专一性抗血清的主要因素
- 人梅毒螺旋体IGM抗体(TP IgM-Ab)酶联免疫分析(ELISA)
- 免疫缺陷病的一般特征
- 沉淀反应技术(Precipitation reaction technique)
- ELISPOT原理、步骤和发展
- 经典激活途径
- Determination of the Immunoreactivity of Radiolabeled Monoclonal Antibodies
- Current Status of Liver Transplantation
- Construction of LPS Mutants
- Comparison of Immunotoxins Bearing a Single Saporin Molecule with Multiple Toxin Conjugates