Multiple Immunofluorescence Analysis of Cells Using Flow Cytometry
As indicated in Chapter 33, flow cytometry has developed rapidly to provide a powerful means of characterizing complex cell populations, both in terms of quantitative analysis of functional cell-associated molecules, and, as is considered here in more detail, the simultaneous analysis of combinations of markers that can be used to identify functional subpopulations of cells. Many of the considerations discussed in the previous chapter are relevant, but issues particularly pertinent to this type of analysis relate to the independence of the markers used, both at the level of the labeling process and at the level of cytometric analysis.
- HLA分子参与T细胞发育和参与黏膜免疫
- 人乙型肝炎病毒前S1抗原(HBV preS1Ag)酶联免疫分析(ELISA)
- 通过抑制信号途径调控细胞因子的激活
- 免疫组织化学技术(immunohistochemistry)
- 佐剂的制备和作用
- The Application of PET Imaging in Psychoneuroimmunology Research
- Soluble Fc Fusion Proteins for Biomedical Research
- Quantitating Lymphocyte Programmed Cell Death In Vitro Using Simple Kill Assays
- Production of CD4+ and CD8+ T Cell Hybridomas
- PAMPs and DAMPs in Allergy Exacerbation Models