Immune Libraries from Nonhuman Primates (NHP)
Libraries constructed from immunised non human primates (NHP; macaques here) are an appealing alternative to human libraries when immunization of Humans is difficult, as is so often the case. These phage-displayed libraries allow to obtain antibody fragments of very high affinities (nano- to picomolar), and diverse epitopes are targeted so that antibodies with the desired activity can be isolated. Due to their high similarity with their human counterparts, NHP antibodies might be readily humanized or germline-humanized, to ensure an excellent tolerance for medical use. Practical peculiarities of this convenient approach, as well as legal aspects, will be considered.
- 免疫印迹法(immunobiotting test,IBT)
- 免疫球蛋白分子
- 疫苗的传递系统
- 免疫接种(immunization)
- The Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoating
- SAGE Analysis of Cell Types Involved in Tolerance Induction
- Methods for In Vitro Generation of Human Type 1 Regulatory T Cells
- Macrophage Depletion by Clodronate
- Interspecies Heterokaryon Assay to Characterize the Nucleocytoplasmic Shuttling of Herpesviral Proteins
- Detection of Soluble CR3 (CD11b/CD18) by Time-Resolved Immunofluorometry