Imaging Interactions Between the Immune and Cardiovascular Systems In Vivo by Multiphoton Microscopy
Several recent studies in immunology have used multiphoton laser-scanning microscopy to visualise the induction of an immune response in real time in vivo. These experiments are illuminating the cellular and molecular interactions involved in the induction, maintenance and regulation of immune responses. Similar approaches are being applied in cardiovascular research where there is an increasing body of evidence to support a significant role for the adaptive immune system in vascular disease. As such, we have begun to dissect the role of T lymphocytes in atherosclerosis in real time in vivo. Here, we provide step-by-step guides to the various stages involved in visualising the migration of T cells within a lymph node and their infiltration into inflamed tissues such as atherosclerotic arteries. These methods provide an insight into the mechanisms involved in the activation and function of immune cells in vivo.
- 补体系统
- b型流感嗜血杆菌结合型疫苗
- 玫瑰花环试验(erythrocyte rosette test)
- 酶的区域性甲基化特异性分析(ERMA)
- 人内皮素 (ET)酶联免疫分析
- western blotting的一些细节-1
- Protocol for Diversion of a CD4+Response to theT-Helper 2 Cell Pathway
- Methods for Assessing the Structure and Function of Cationic Antimicrobial Peptides
- General Fusion Protocol
- Conjugation of Fluorochromes to Antibodies