Identification of Target and Function Specific Antibodies for Effective Drug Delivery
Phage antibody technology is a powerful approach for generating human antibodies to virtually any target antigen. For many therapeutic applications, it is useful to generate antibodies that bind to cell-surface receptors in a manner where binding results in internalization of the antibody. This allows use of the antibody to deliver toxic payloads intracellularly to achieve a therapeutic effect. Here we describe how phage antibody libraries can be directly selected on tumor cell lines to generate antibodies binding cell-surface receptors and which are rapidly internalized upon binding. Protocols are provided showing how to (1) directly select internalizing antibodies from phage antibody libraries; (2) screen phage antibodies in a high-throughput flow cytometry assay for binding to the tumor cell line used for selection; (3) identify the antigen bound by the phage antibody using immunoprecipitation and mass spectrometry; and (4) verify and quantitate such that phage antibodies are internalized.
- 免疫球蛋白分子的结构与功能
- 细胞因子补充和添加疗法
- 细菌的生理、生化试验简介
- 人工自动免疫(artificial active immunity)
- 肿瘤坏死因子(TNF)
- 肿瘤基因治疗临床试验最常用的几种策略
- Flow Cytometric Methods of Analyzing Apoptotic Cells
- CD17分子
- CD13 and CD10 Expression of Granulocytes as Markers for the Functioning of the Immune System: Quantification of the Expression o
- Bispecific Monoclonal Antibodies for the Targeting of Type I Ribosome-Inactivating Proteins Against Hematological Malignancies