Hydrodynamic Delivery Protocols
RNA interference (RNAi) holds considerable promise as a novel therapeutic strategy to silence disease-causing genes not amenable to conventional therapeutics. Since it relies on small interfering RNAs (siRNAs), which are the mediators of RNAi-induced specific mRNA degradation, a major issue is the delivery of therapeutically active siRNAs into the target tissue. In vivo gene silencing with RNAi has been reported using both viral vector delivery and high-pressure, high-volume intravenous (i.v.) injection of synthetic siRNAs. For safety reasons, strategies based on viral vector delivery may be only of limited clinical use. The more desirable approach is to directly deliver active siRNAs. We describe the use of hydrodynamic administration as a technique to deliver naked siRNA constructs into experimental animals as a method of transient gene knockdown. This approach demonstrates that RNAi can be used to silence endogenous genes, involved in the cause of human diseases, with a clinically acceptable formulation and route of administration.
- 多肽疫苗
- 生物芯片的主要种类
- 免疫斑点试验(Dot Immunobinding Assay, DIBA)
- 用ELISpot检测人类HLA2Ⅰ抗体的方法
- 流式细胞仪的构造与原理
- Purification, Preparation, and Use of ChaperonePeptide Complexes for Tumor Immunotherapy
- HLA-DPA1 and -DPB1 Typing Using the PCR and Nonradioactive Sequence-Specific Oligonucleotide Probes
- General Fusion Protocol
- Determination of Fc-Mediated Antibody-Effector Functions by Chromium Release Assay
- Detection Methods for Glycopeptide-Resistant Staphylococcus aureus II: Cell Wall Analysis