How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells Secretory Activity
Over the past decade, ELISPOT has become well-established as a mainstream technology for the study of immune responses in vivo mainly due to its unique ability to detect rare antigen-specific lymphocytes ex vivo. The primary readout for ELISPOT assays has traditionally been the measurement of the frequency of analyte-secreting cells within a test population. While it has been generally appreciated that ELISPOT is a high-information-content assay system in which spot morphologies provide additional valuable information on the amount of analyte secreted by individual cells as well as the kinetics of the secretory process, the precise relationships involved have not been fully characterized and the specific relevant information �conveyed by spot morphologies has remained largely unexplored. In an attempt to bridge this gap, we formulated an in silico kinetic model for spot formation and derived a solution for the model in both a general and a numerical form. Both solutions suggested a logarithmic relationship between spot size and cell productivity. This chapter involves an in-depth analysis of the relationship between observed spot morphologies and cells’ secretory functions (as well as an examination of additional assay parameters), and predictions based on the mathematical model are verified under experimental assay conditions where possible.
- B细胞亚群
- HLA分子参与T细胞发育和参与黏膜免疫
- 各种组织切片方法的操作步骤和应用范围
- 构成抗原的条件
- 造血干细胞的特性
- Solid Phase Assay Measuring C4d Deposition to Determine Complement Fixation by HLA-Specific Antibodies
- Molecular Cloning of Antiground-State Proteolytic Antibody Fragments
- HIV-1 Chromatin, Transcription, and the Regulatory Protein Tat
- Expression of Chemokines in Escherichia coli
- Expression of Cell Surface Antigens on Mast Cells: Mast Cell Phenotyping