Detection of SARS Coronavirus
The emergence of severe acute respiratory syndrome (SARS) and its subsequent worldwide spread challenged the global public health community to confront a novel infectious disease. The infection is caused by a coronavirus of animal origin. In this epidemic, molecular detections of SARS coronavirus RNA were shown to be useful for the early diagnosis of SARS. Although this pathogen was eradicated in humans, SARS or SARS-like viruses might reemerge from animals or from laboratory incidents. In this chapter, we describe several polymerase chain reaction (PCR) protocols for detecting SARS coronaviruses. These assays were routinely used for clinical diagnosis during the SARS outbreak.
- ArrayStar转录因子活性ELISA检测法
- 抗原制备
- 利用HLA/抗原肽四聚体和流式细胞分选仪制备CTL细胞克隆
- 关于ELISPOT培养板的选用问题
- 小鼠乙酰胆碱 (ACh)酶联免疫分析(ELISA)
- Three Methods to Assay Inhibitors of Ribosomal Subunit Assembly
- MicroRNA-Profiling in Formalin-Fixed Paraffin-Embedded Specimens
- Methods to Determine Antibiotic Resistance Gene Silencing
- Isolation of RNA and the Synthesis and Amplification of cDNA from Antigen-Specific T Cells for Genome-Wide Expression Analysis
- CD30分子