Welcome to MethPrimer

• MethPrimer is a program for designing bisulfite-conversion-based Meth ylation PCR Primer . Currently, it can design primers for two types of methylation mapping PCRs: 1) Methylation-Specific PCR (MSP) and 2) Bisulfite-Sequencing PCR (BSP) or Bisulfite-Restriction PCR.
• Input Sequence : DNA sequences in any format. No editing is required before input.
• Output : MethPrimer returns results in both text and graphic view.

How to cite MethPrimer : Li LC, Dahiya R. . Bioinformatics. 2002 Nov;18(11):1427-31. (moderated) Join the CpG method forum to ask questions and share your knowledge on methylation related  methods, such as bisulfite modification, primer design, MSP, bisulfite sequencing PCR. Acknowledgement: MethPrimer is based on on the well-known primer design program (Steve Rozen, Helen J. Skaletsky (1998) Primer3. Code available at   Copyright (c) 1996,1997,1998 Whitehead Institute for Biomedical Research. All rights reserved. ).  MethPrimer was developed by Dr. at Dr. , Veterans Affairs Medical Center and University of California, San Francisco.