Bioreactor Production of scFv Fragments in Pichia pastoris
The pubmed search term “pastoris[Title] AND (express[Title] OR produced[Title] OR expression[Title] OR production[Title]) ” yielded 877 hits in December 2008, dated from 1987 to 2009. At the same time, the search term “pastoris[Title] AND (bioreactor[Title] OR fed-batch[Title] OR continuous[Title] OR fermentations[Title] OR large-scale[Title] OR fermentation[Title] OR pilot[Title]) ” returned 92 hits –published between 1990 and 2009. This analysis is somewhat superficial and ostentatious, but it suggests that the majority of researchers publishing on Pichia use it as a tool for rather than an object of their work. This is not to say that the majority should change their focus, but in fact researchers sometimes face difficulties when the need to obtain useful amounts of a target protein produced in Pichia calls for scale-up from the benchtop protocols to a bioreactor-based process. This chapter attempts to provide a reliable protocol for AOX1 -driven bioreactor production of secreted scFvs or other proteins.
- 人Caspase-3(Caspase-3)ELISA试剂盒 说明书
- 人半胱氨酸(CYS)ELISA试剂盒 说明书
- 杀伤细胞凝集素样受体(KLR)
- 夹心法ELISA检测可溶性白介素2受体实验方法
- 各种组织切片方法的操作和优缺点分析
- Ⅲ型变态反应(Ⅲtypeallergy)
- RNA Purification and Expression Analysis Using Microarrays and RNA Deep Sequencing
- Nonradioactive Methods for Visualization of Protein Blots
- Heat-Induced Antigen Retrieval for Immunohistochemical Reactions in Routinely Processed Paraffin Sections
- Biomarkers Measuring the Activity of Toll-Like Receptor Ligands in Clinical Development Programs