Purification and Enzymatic Sequencing of Polymerase Chain Reaction Products
The advent of direct sequencing of polymerase chain reaction (PCR) products has permitted extremely rapid analysis of DNA mutants and cDNA clones. However, direct PCR sequencing has been problematic for a number of technical reasons, including the presence of impurities and excess oligonucleotide primers used for the PCR amplifications (1 –4 ). Therefore, a number of protocols have been devised that address these technical issues, and allow efficient sequencing of either conventional double-stranded PCR products or asymmetrically amplified single-stranded products (e.g., 1 –4 ). Many of these protocols are described in detail in this volume.
- 人HBV PreS2酶联免疫分析(ELISA)
- 铅蓄电池
- Gene Therapy Techniques for the Delivery of Endothelial Nitric Oxide Synthase to the Corpora Cavernosa for Erectile Dysfunction
- Lipases Production by Solid-State Fermentation: The Case of Rhizopus homothallicus in Perlite
- 弥散性血管内凝血的原因和发病机制
- 聚丙烯酰胺凝胶电泳鉴定IgG纯度
- Transient transfection into 293T cells
- 配位化合物-- 配合物的基本概念
- In Vitro Continuation of RNA Synthesis Initiated in Vivo
- 生态平衡