In Vitro Translation of mRNA in a Rabbit Reticulocyte Lysate Cell-Free System
The identification of specific messenger RNA molecules and the characterization of the proteins encoded by them has been greatly assisted by the development of in vitro translation systems. These cell-free extracts comprise the cellular components necessary for protein synthesis, i.e., ribosomes, tRNA, rRNA, amino acids, initiation, elongation and termination factors, and the energy-generating system (1 ). Heterologous mRNAs are faithfully and efficiently translated in extracts of HeLa cells (2 ), Krebs II ascites tumor cells (2 ), mouse L cells (2 ), rat and mouse liver cells (3 ), Chinese hamster ovary (CHO) cells (2 ), and rabbit reticulocyte lysates (2 ,4 ), in addition to those of rye embryo (5 ) and wheat germ (6 ). Translation in cell-free systems is simpler and more rapid (60 min vs 24 h) than the in vivo translation system using Xenopus oocytes.
- Cryopreservation of Mammalian Embryos
- In vitro Analysis of the Mitochondrial Preprotein Import Machinery Using Recombinant Precursor Polypeptides
- 人纤溶酶原激活物抑制因子 (PAI)酶联免疫分析
- 芬兰计划向生物柴油工厂提供 700万欧元的补贴
- 休克的血液流变学变化
- Covalent and Noncovalent Labeling Schemes for Near-Infrared Dyes in Capillary Electrophoresis Protein Applications
- Quality Control of Solid-Phase Synthesis by Mass Spectrometry
- Luminescent Detection of Specific DNA Sequences
- 自由水
- 偶极矩