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化学生物学实验技术>Affinity-Capture and Solid-Phase Sequencing of Biotinylated Polymerase Chain Reaction Products
Affinity-Capture and Solid-Phase Sequencing of Biotinylated Polymerase Chain Reaction Products
关键词: affinity-capture affinity来源: 互联网
The time and labor required for generating nucleotide sequence information has been significantly reduced since the development of the polymerase chain reaction (PCR). PCR allows the production of sufficient amounts of DNA template in vitro, and consequently the time-consuming cloning procedure to obtain large enough quantities of DNA for sequencing is avoided. The PCR has transformed nucleotide sequencing into a method that can be considered even in routine diagnostics for sequencing large numbers of individual samples.
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