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化学生物学实验技术>A Nonradioactive Assay for Nitric Oxide Synthase Activity in Tissue Extracts
A Nonradioactive Assay for Nitric Oxide Synthase Activity in Tissue Extracts
关键词: nonradioactive assay来源: 互联网
We describe a fluorescence assay for nitric oxide synthase activity based on a new indicator, 4,5-diaminofluorescein (DAF-2). The method offers the advantage of being safer and more convenient than the citrulline radioassay in common use. The rapid and irreversible binding of DAF-2 to oxidized nitric oxide (NO) enables NO production to be measured in real time. The protocol is applied to the measurement of nitric oxide synthase in crude extracts of skeletal muscle.
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