DNA文库的构建及其筛选
实验方法
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Yeast Two-Hybrid Screen with Library and Bait
YEAST TWO-HYBRID SCREEN WITH LIBRARY AND BAIT(The following protocol is for use with the LIBRARY tra
来源:互联网 关键词:Two-Hybrid
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Screening lambda cDNA or genomic libraries
MediaNeed LB plates (9 cm or 14 cm)to plate the library and do secondary screens.I typically screen
来源:互联网 关键词:
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再扩增VH基因文库添加3‘限制性位点并克隆创建VH基因节段文库
[器材和试剂]● PCR试剂和设备● Wlzard PCR纯化试剂盒(Promega)● PVHlOR2引物: 5'-GAG TCA TTC TCG ACT TGC GGC CGC TCG C
来源:互联网 关键词:再扩增VH基因文库 限制性位点 克隆 VH基因节段文库