The emphasis of this work is on the application of 9-AA on lipidomic analysis as we have recently conducted. We have found that 9-AA as matrix for MALDI-MS analysis of lipids offers a number of advantages over essentially all other matrix used in the literature, including minimal matrix background, reduced post-source decay, enhanced ionization efficiency (particularly in the negative-ion mode),and increased sensitivity for certain lipid classes (e.g.,sulfatide). We have also demonstrated that many classes of lipids can be directly analyzed from a lipid extract of a biological sample and the spectra acquired by MALDI-MS with 9-AA as matrix are comparable with those obtained from electrospray ionization MS which has recently been used as a “golden standard” for quantitative analysis of lipids. Collectively, the recent developments of MALDI-MS provide new sprints for lipidomic research.