MEDIUM FOR EMBRYO RESCUE (FOR 1 L)
Put 500 mL of distilled water in a 1-L beaker.
Add the following:
ucrose (6 % final concentration) – 30 g
i-inositol (100 mg/L stock is 0.5 g/50 mL) – 10 mL
Thiamine (0.4 g/l stock is 2 g/50 mL) – 10 mL
Amino acids L-tyrosine, L-argenine, glycine – 10 mL (10 mg/L stock is 50 mg each aa/50 mL H2O)
Kinetin (for very small enbryos only) – 2 mg
Muraschige–Skoog Organic medium – 34.6 g
NaH2PO4H2O (stock 17 g/L) – 10-mL stock
Inorganic salts
Organic constituents
Dilute to 800 mL.
Adjust pH to 5.7 with NaOH..
Bring volume to 1,000 mL.
Add 8 grams Bacto® agar.
Melt agar in microwave for 3 minutes or dissolve while stirring on a hotplate.
Pour ~20 mL into Kimax culture tubes (50 mL) and cap.
Sterilize by autoclaving for 15 minutes.
Slant in tubes or pour into petri dishes.
