使用三重串联四极杆液
Abstract: 基于安捷伦 1200高分离快速液相色谱(RRLC)/组合离子源的 6430A三重串联四极杆液质联用系统开发了血清中
25-OH D2 和 25-OH D3 的快速定量分析法。 固相萃取最大限度降低了基质干扰和离子抑制，同时还能自动化运行。在 5至 100 ng
/ mL的浓度范围内具有优异的定量线性度响应(R2 > 0.99)，而且分析时间只有五分钟，日间精密度远远低于
10%。血清中的检测限(LOD)和定量限(LOQ)分别是 2 ng/mL 和 3 ng/mL。 A rapid method for the
quantification of 25-OH D2 and 25-OH D3 in serum was developed using the
Agilent 1200 Series Rapid Resolution LC (RRLC) system coupled with an
Agilent 6430 Triple Quadrupole LC/MS with multimode ionization (MMI).
Solid phase extraction minimizes matrix interferences and ion
suppression, while enabling automation. Excellent linearity of
quantification (R2 > 0.99) was obtained in the range of 5 to 100
ng/mL, with run times of only 5 min, and day-to-day precision was well
below 10%. The limits of detection (LOD) and limits of quantification
(LOQ) were 2 ng/mL and 3 ng/mL respectively, in serum.
Keywords: 1200，快速高分离液相色谱，多模式进样口，血液，癌症，钙，磷 1200, RRLC, 6430, MMI, blood, cancer, calcium, phosphorus
Publication Number: 5990-4549CHCN
Last Updated: 9/3/2010
Number of Pages: 4
View Article (0.47 MB)
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