线粒体荧光探针大全：TMRM，Mitotracker，JC-1（6）
Pyruvate Dehydrogenase
Molecular Probes has available a series of antibodies against the human pyruvate dehydrogenase (PDH) complex (Table 12.6), a large, multienzyme assembly residing in the mitochondrial matrix and consisting of three catalytic activities: pyruvate dehydrogenase, dihydrolipoyl transacetylase and dihydrolipoyl dehydrogenase 193 (diaphorase). The PDH complex is responsible for the oxidative decarboxylation of pyruvate to form acetyl coenzyme A, which is in turn fed into the citric acid cycle. Deficiencies in the PDH complex lead to lactic acidosis;194severe cases can lead to developmental defects such as congenital brain malformation.195 In addition to unlabeled subunit-specific anti-PDH antibodies, we offer the red-fluorescent Alexa Fluor 594 conjugate of anti–PDH E1
subunit antibody (A31853), as well as the green-fluorescent Alexa Fluor 488 conjugate of anti–PDH E2 subunit antibody (A31854).
Mitochondrial Protein Extracts
For researchers seeking a source of mitochondrial protein standards, Molecular Probes offers human heart mitochondrial proteins for SDS-polyacrylamide gel electrophoresis (M22430). This complete mitochondrial lysate has tested negative for hepatitis B and C, as well as HIV 1 and 2 in serology tests. Mitochondrial protein extracts are useful for comparing new mitochondrial protein preparations in SDS-polyacrylamide gels and for testing mitochondrial antibodies.
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