45种培养基配方（细菌培养基与植物培养基） （三）
19. Filter Paper Medium （滤纸培养基）
（NH4）2SO4 1g KH2PO4 1g
MgSO4.7H2O 0.7g NaCl 0.5g
Distilled water （蒸馏水） 1000ml A strip of filter paper （滤纸条） 6ⅹ1cm （厘米）
20. Soybean Cake Meal Agar (黄豆饼粉琼脂)
10% infusion of soybean cake meal (10%黄豆饼粉浸汁) 1000ml
Glucose (葡萄糖) 10g NaCl 2.5g
21. Sawdust， Wheat-bran Medium （木屑、麸皮培养基）
Sawdust of broad leaf tree 75g Wheat bran 25g
Preparation：Mix these two ingredients well . Add appropriate water and blend
(the amount of water added may be becided by kneading with fingers ). Fill the
mixture into broad mouth of bottles . Stick a hole on the filling, put the
cotton pulg in bottle mouth .
壳斗科植物或阔叶树的木屑 75克 麸皮 25克
制法：加水适量充分混匀，水量以手捏时指间有水珠为宜，装瓶并压紧，在中心处扎一小洞，堵上棉塞，20磅灭菌1小时。
22. Pine Block or Pine Sowdust Medium （松木条或松木屑培养基）
1. Cut 1021 cm pine biocks and immerse them in 1-2% sucrose solution . Alow
the biocks fully absorbing suger solution .Fill the biocks into broad month of
bottle . Park the bottle with paper . Autoclave at 20 lbs for 1 hr .
(将10ⅹ2ⅹ1厘米的松木条浸泡在1-2%的蔗糖水中。使木条吸足糖水，装瓶不宜过紧，用纸包好，20磅灭菌1小时。)
2. Pine Sawdust Medium
Pine Sawdust (松木屑) 75% Rice bran (米糠) 23-24%
Socrose (蔗糖) 1-2% Water (appropriate)水 （适量）
Autoclave at 20 lbs for 1 hr.(20磅灭菌1小时）
23. Rice Straw. Dung Medium (稻草、马粪培养基)
Dried horse dung (or cow dung) （干马粪或牛粪） 65%
Died straw （干稻草） 35% Urea （尿素） 0.5%
Calcium superhosphate （过磷酸钙）0.7% Gypsum powder（石膏粉） 1%
Ammonium sulphate （硫酸铵） 0.3%
[Note]: Add appropriate water, pile them up and cause them fermentation.
（水适量进行堆积发酵）
24. Straw Rice Bran Medium （稻草、米糠培养基）
Dried straw （干稻草） 75-80% Rice bran （米糠） 20-25%
[Note]：Select no mouldy straw and cut them about 3 cm in length. Immerse them
in fresh water for 12 hr. Allow the straw fully (the amount of water added may
be decided by kneading with fingers. It is right when a drop of water appeared
between fingers). (
[注]：选无霉稻草，切成长约3厘米的小段，用清水浸泡12小时，使充分吸水，然后倾去水，加米糠和水，水量以用手捏时指间有水珠为宜。)
25. Rice Medium (米饭培养基)
Fill 10g of rice into a 50ml of Erlenmeyer flask. Add 20-30ml water. Autoclave
at 121℃ for 30 minutes (or fill 2g of rice into a 15ⅹ150mm tube). Add 6 ml of
water. Autoclave at 121℃ for 30 minutes.
(取大米10克，入50毫升椎形瓶中，水20-30毫升于15磅灭菌30分钟即成，或取大米2克，装入15ⅹ150毫米试管中，加水6毫升，于15磅灭菌30分钟。)
26. Filter Paper Medium (滤纸条培养基)
(NH4)2SO4 1g KH2PO4 1g
MgSO4.7H2O 0.5g Yeast Extract (酵母膏) 0.1g
Distilled Water (蒸馏水) 1000ml
Strip of filter paper 1ⅹ7 cm (滤纸条 1ⅹ7 厘米)
[Note]：Siddolve the above ingredients. Distribute the liqid medium into tubes
(each tube contains 1ml of the broth). Autoclave at 121℃ for 30 minutes. Then
put one or two sterilized strips of filter paper into a tube aseptically.
(将上述溶液分配成溶液后分装入试管，每管1毫升，15磅灭菌30分钟。再将灭菌的滤纸条（1条或2条）投入试管中即好使用。)
27. Yeast Extract-Medium Extract Agar I (酵母膏、麦芽膏琼脂)
Yeast extract (酵母膏) 10g Mall extract (麦芽膏) 10g
Glucose (葡萄糖) 4g Distilled water (蒸馏水) 1000ml
Agar (琼脂) 20g pH 7.0
适用范围：红霉素链霉菌
