关注公众号

关注公众号

手机扫码查看

手机查看

喜欢作者

打赏方式

微信支付微信支付
支付宝支付支付宝支付
×

如何用imagej分析免疫组化平均光密度值

2022.9.20

1、打开Image-Pro Plus 6.0软件,点击右下方的Done。点击file,点击open,打开你要选择组化图片,点击打开,得到如图所示。

11385343fbf2b211c9b04a32da8065380cd78e34?x-bce-process=image%2Fresize%2Cm_lfit%2Cw_600%2Ch_800%2Climit_1%2Fquality%2Cq_85%2Fformat%2Cf_auto

2、点击file那一排的measure,选择calibration右侧下方的intensity。点击OD旁边的new,点击std. optical density,点击options,

e61190ef76c6a7ef4cb8675aedfaaf51f3de6634?x-bce-process=image%2Fresize%2Cm_lfit%2Cw_600%2Ch_800%2Climit_1%2Fquality%2Cq_85%2Fformat%2Cf_auto

3、点击255旁边的image,弹出如图所示对话框,点击图中空白地方,点击ok键。将incidental level改成255。

c8177f3e6709c93dc46cc18b8f3df8dcd1005434?x-bce-process=image%2Fresize%2Cm_lfit%2Cw_600%2Ch_800%2Climit_1%2Fquality%2Cq_85%2Fformat%2Cf_auto

4、继续点击ok,点红色x,关闭。点击measure→count/size。

50da81cb39dbb6fd83c52a5a1924ab18972b3734?x-bce-process=image%2Fresize%2Cm_lfit%2Cw_600%2Ch_800%2Climit_1%2Fquality%2Cq_85%2Fformat%2Cf_auto

5、点击selecte colors,点击最左边像笔的图标,选免疫组化图片阳性蛋白表达区域,选择完毕,点击close。

b21c8701a18b87d62cdb6463170828381f30fd35?x-bce-process=image%2Fresize%2Cm_lfit%2Cw_600%2Ch_800%2Climit_1%2Fquality%2Cq_85%2Fformat%2Cf_auto

6、点击表格中的view,选择statistic。得到mean那一栏和iod交叉的数值,就是mean of iod。

7af40ad162d9f2d34b88eb93b9ec8a136327cc35?x-bce-process=image%2Fresize%2Cm_lfit%2Cw_600%2Ch_800%2Climit_1%2Fquality%2Cq_85%2Fformat%2Cf_auto


推荐
关闭