Inflammasomes PCR Array

炎性PCR芯片
 

Product	Species	Technology	Cat. No.
Inflammasomes PCR Array	Human	Gene Expression	PAHS-097Z
Inflammasomes PCR Array	Mouse	Gene Expression	PAMM-097Z
Inflammasomes PCR Array	Rat	Gene Expression	PARN-097Z

The Human Inflammasomes RT² Profiler™ PCR Array profiles the expression of 84 key genes involved in the function of inflammasomes, protein complexes involved in innate immunity, as well as general NOD-like receptor (NLR) signaling. NLRs represent a major class of cytosolic pattern recognition receptors (PRR) that, like their cell-surface Toll-Like Receptor counterparts, recognize a wide variety of microbial pathogens and immunogenic biological products. Activation of one of four PRR family members (AIM2, NLRC4 or IPAF, NLRP1, and NLRP3) initiates the formation of an inflammasome. These protein complexes in turn activate caspase-1, leading to up-regulation of the pro-inflammatory cytokines IL1B and IL18 and pyroptosis, or caspase-1-dependent programmed cell death. This array includes genes encoding inflammasome components as well as genes involved in downstream signaling and inhibition of inflammasome function. In addition, this array includes other NLR family members, which may potentially form additional inflammasomes, and their downstream signaling genes. Using real-time PCR, you can easily and reliably analyze the expression of a focused panel of genes involved in inflammasome and NLR function and signaling with this array.
炎性PCR芯片检测84个关键基因的表达，包括炎性体相关基因，先天免疫的蛋白复合物，以及一般的NOD样受体（NLR）信号基因。NLRs是一类主要的胞浆内模式识别受体（PRR），与细胞表面Toll样受体一样，识别多种多样的病原微生物和免疫原性生物产物。PRR四个家庭成员（AIM2、NLRC4/IPAF、NLRP1和NLRP3）中任何一个的激活，都将引起炎性体的形成。而这些蛋白质复合物又反向激活caspase-1，使促炎性细胞因子IL1B、IL18和pyroptosis上调，从而导致炎症坏死，以及caspase-1依赖的程序性细胞死亡。芯片包括编码炎性体组件的基因、炎性体功能发挥的下游信号和抑制基因、其他NLR家庭成员，以及可能形成更多的炎性体和其下游信号的基因。通过实时定量PCR的方法，研究者即能够利用该芯片简单可靠地同时检测炎性体和NLR功能和信号相关的基因表达.
Inflammasomes:
AIM2: AIM2, CASP1 (ICE), PYCARD (ASC).
IPAF: CASP1, NAIP, NLRC4 (IPAF), PYCARD (ASC).
NLRP1: CASP1 (ICE), CASP5, NLRP1.
NLRP3: CASP1 (ICE), NLRP3, PYCARD (ASC).
Negative Regulation: BCL2, BCL2L1 (BCL-X), CARD18 (ICEBERG), CD40LG (TNFSF5), CTSB, HSP90AA1, HSP90AB1, HSP90B1 (TRA1), MEFV, PSTPIP1, PYDC1 (POP1), SUGT1, TNF, TNFSF11, TNFSF14, TNFSF4 (OX40L).
Downstream Signaling: IFNG, IL12A, IL12B, IL18, IL1B, IL33, IRAK1, IRF1, MYD88, P2RX7, PANX1, PTGS2 (COX2), MOK, RIPK2, TIRAP, TXNIP.
NOD-Like Receptors:
Receptors: CIITA, NAIP (BIRC1), NLRC4 (IPAF), NLRC5, NLRP1, NLRP12, NLRP3, NLRP4, NLRP5, NLRP6, NLRP9, NLRX1, NOD1 (CARD4), NOD2.
Downstream Signaling: BIRC2 (c-IAP2), BIRC3 (c-IAP1), CARD6, CASP8 (FLICE), CCL2 (MCP-1), CCL5 (RANTES), CCL7 (MCP-3), CFLAR (CASPER), CHUK (IKKa), CXCL1, CXCL2, FADD, IFNB1, IKBKB, IKBKG, IL6, IRF1, IRF2, MAP3K7 (TAK1), MAPK1 (ERK2), MAPK11, MAPK12, MAPK13, MAPK3 (ERK1), MAPK8 (JNK1), MAPK9 (JNK2), NFKB1, NFKBIA (I?Ba/MAD3), NFKBIB (TRIP9), PEA15, RELA, RIPK2, SUGT1, TAB1 (MAP3K7IP1), TAB2 (MAP3K7IP2), TNF, TRAF6, XIAP.
Pro-Inflammatory Caspases: CASP1 (ICE), CASP5