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Grow at 37°C with shaking to an OD600 of 0.5-1.0 (should take 3-6 hr). Induce expression of the protein by adding IPTG to a final concentration of 0.1 mM. ...
期刊链接:http://www3.interscience.wiley.com/cgi-bin/jhome/26737期刊简介:Angewandte Chemie International Edition, with its excellent Impact Factor of 10.232 (2006) strengthens its leading position among the general...
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