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one sample duplicated in the other piece. 3) Place the two pieces in separate containers of the CIP buffer, 3-5 ml per container. 4) Add CIP to the container with the piece to be de-phosphorylated...
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.2) To this solution add 10ul of 10 x OPA+ and 1ul of a 0.1 unit/ul of CIP in 1 x OPA+.N.B: Do not use more that 0.1 units CIP if it can be avoided as inactivation of CIP by heat is not 100% effective...
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