This document specifies safety requirements and their verification for the design and construction of power take-off (PTO) drive shafts and their guards linking a tractor or self-propelled machinery to the first fixed bearing of recipient machinery. It describes methods for the elimination or reduction of risks which need specific requirements including such risks arising from misuse, reasonably foreseeable by the manufacturer. It is applicable only to those PTO drive shafts and guards mechanically linked to the shaft by at least two bearings. In addition, it specifies the type of information on safe working practices to be provided by the manufacturer. This document does not deal with: - the guards totally covering, but not mechanically linked to, the PTO drive shaft; - the mechanical characteristics of PTO drive shafts, overrun devices and torque limiters; - general hazards which are dealt with in EN ISO 4254-1:2015 (see introduction). Environmental aspects have not been considered in this document. This document is not applicable to PTO drive shafts and their guards which are manufactured before the date of publication of this document by CEN.