Full Description ADOPTED_FROM:EN ISO 13365-2:2020 This document specifies a test method by artificial perspiration solution aqueous extraction for the determination of the aqueous extractable content of the following preservative agents in leather by liquid chromatography: — 2-(thiocyanomethylthio)-benzothiazole (TCMTB); — 4-chloro-3-methylphenol (PCMC); — 2-phenylphenol (OPP); — 2-octylisothiazol-3(2H)-one (OIT); This method can also be used to determine breakdown products of these preservative agents, which protect leather from microbiological attack.