1 Scope
This document describes the characteristics of working suspensions of nano-objects to be considered when conducting in vitro assays to evaluate inherent
nano-object toxicity. In addition, the document identifies applicable measurement
methods for these characteristics.
This document is applicable to nano-objects, and their aggregates and agglomerates
greater than 100 nm.
This document intends to help clarify whether observed toxic effects come from tested
nano-objects themselves or from uncontrolled sources.