1 Scope
This document specifies a method for the determination of primary, secondary and tertiary aliphatic and aromatic amines in indoor air using accumulated sampling and high-performance liquid-chromatography (HPLC) coupled with tandem mass spectrometry (MS-MS) or high resolution mass spectrometry (HRMS). It specifies the sampling procedure for determining the mass concentration of amines as mean values by sampling the amines on phosphoric acid impregnated filters. The analytical procedure of the measurement method is covered by ISO 16000‑39.
Measurements, performed with samplers containing phosphoric acid-impregnated inert supporting material and operating at specified flow rates for specified sampling periods are described in this document. Requirements regarding sample volume are also defined.
The range of application of this document concerning the concentrations of amines in indoor air depends on the linear range of the calibration line and hence on the gas sample volume (here: from 5 l up to 100 l), the eluate volume (from 1 ml up to 5 ml), the injection volume (from 1 µl up to 10 µl) and the sensitivity of the analytical equipment (e.g. linear range from 2 pg up to 2 ng amine). The range of application can be expected to be from approximately 0,002 µg/m 3 (100 l sample) up to 2 000 µg/m 3 (5 l sample) for a common analytical equipment 1 for the majority of the amines listed in Annex A. The analysis of derivatives of ethanolamine is usually about 10 times more sensitive and the analysis of short-chained aliphatic amines is usually about 10 times less sensitive than the analysis of an avera...