EN ISO 19040-3:2022
水质.水和废水雌激素潜能的测定.第3部分：体外人细胞报告基因测定

Water quality - Determination of the estrogenic potential of water and waste water - Part 3: In vitro human cell-based reporter gene assay (ISO 19040-3:2018)

作废

EN ISO 19040-3:2022 发布历史

EN ISO 19040-3:2022由欧洲标准化委员会 IX-CEN 发布于 2022-09-21，并于 2022-12-31 实施，于 2023-03-31 废止。

EN ISO 19040-3:2022在国际标准分类中归属于: 13.060.70 水的生物学特性的检验。

EN ISO 19040-3:2022的历代版本如下：

• 2022年 EN ISO 19040-3:2022 水质.水和废水雌激素潜能的测定.第3部分：体外人细胞报告基因测定

This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay utilizing stably transfected human cells. This reporter gene assay is based on the activation of the human estrogen receptor alpha. This method is applicable to: — fresh water; — waste water; — aqueous extracts and leachates; — eluates of sediments (fresh water); — pore water; — aqueous solutions of single substances or of chemical mixtures; — drinking water; — the limit of quantification (LOQ) of this method for the direct analysis of water samples is between 0,3 ng/l and 1 ng/l 17β-estradiol equivalents (EEQ) based on the results of the international interlaboratory trial (see Annex F). The upper working range was evaluated [based on the results of the international interlaboratory trial (see Table F.3)] up to a level of 75 ng EEQ/l. Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre concentration of water samples can prove necessary if their estrogenic potential is below the given LOQ.

标准号

EN ISO 19040-3:2022

发布

2022年

发布单位

欧洲标准化委员会

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EN ISO 19040-3:2022

 

 

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