GSO ISO 11063:2013由GSO 发布于 2013-04-01。
GSO ISO 11063:2013在国际标准分类中归属于: 13.080.30 土壤的生物特性。
GSO ISO 11063:2013 土壤质量 从土壤样品中提取 DNA 的直接方法的最新版本是哪一版?
最新版本是 GSO ISO 11063:2013 。
This International Standard specifies a method for direct extraction of DNA from soil samples to analyse the global structure and the abundance of soil bacterial communities using PCR-based technologies. This method is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals. The direct extraction of DNA from soil samples provides unique insight into the richness and structure of microbial communities which are key parameters to estimate the biodiversity of soil microbiota. Molecular approaches based on PCR (polymerase chain reaction) amplification of soil DNA constitute a promising domain and can contribute in the near future to the development of routine tools to monitor the microbiota of soil environments. Users of the method ought to be aware that although soil submitted to the DNA extraction procedure is sieved thoroughly (2 mm mesh, procedure described in 5.1), plant residues can still remain in soil samples and, as a result, traces of plant DNA can contaminate the soil DNA extract.
目前从土壤样品中提取微生物DNA的方法主要有Bead beating法、Bourrain法、Martin-Laurent法,而这些方法都统分为直接法和间接法。直接法是指将土壤样品放在裂解液中,经过有效的破壁方法使微生物的DNA全部释放到裂解液中,然后再进行分离提取的方法。间接法是指将土壤放在一种缓冲液中,把微生物从土壤中分离出来,然后再进行DNA的提取。...
OMNI BeadRuptor 24 Elite研磨珠均质器应用于土壤样品中DNA提取应用笔记Omni国际土壤DNA试剂盒优于公司M土壤DNA分离试剂盒,用于从土壤样品中提取DNAOmni国际有限公司Shari Garrett介绍土壤DNA的分子分析是检测土壤微生物和微生物多样性的直接解决方案。...
Volossiouk[3]等使用液氮研磨及SDS(十二烷基磺酸钠)破解微生物细胞提取土壤DNA,Tsai[4]等则采用溶菌酶和SDS法提取土壤DNA,而这些方法所提取的DNA杂质较多,严重影响后续研究结果[5-6]。后来,Bourrain等[7]从活性污泥中以及LaMontagne等[8]从堆肥中提取了微生物群落的DNA。但是,目前仍然没有一种快速、高效、低成本且高质量的土壤DNA提取方法。...
土壤样品含有大量的微生物,其中绝大部分的微生物都是无法直接培养进行繁殖和研究。从土壤样品中提取 DNA 是研究土壤微生物zui为有效的方法。目前从土壤样品中提取微生物 DNA 的方法主要有直接法和间接法。直接法是指把土壤样品放在裂解液中,经过有效的破壁方法使微生物的DNA 全部释放到裂解液中,然后再进行分离提取,如 Zhou 的方法。...
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