These test methods cover procedures for determining penetration of preservatives in wood in cases where demarcation between the treated and untreated wood is not readily visible. Included are test methods for differentiating the heartwood and the sapwood of wood samples for specific species, and a test method for differentiating the heartwoods between the red oaks and the white oaks. The procedures appear in the following order: Procedure Sections Penetration of Arsenic-Containing Preservatives 6 to 8 Penetration of Copper-Containing Preservatives 9 to 11 Penetration of Fluoride-Containing Preservatives 12 to 15 Penetration of Pentachlorophenol Using 4, 4[prime]-bis-Dimethylamino-Triphenylmethane (DMTM) 16 to 20 Penetration of Pentachlorophenol Using a Silver-Copper Complex Known as "Penta-Check" 21 to 24 Penetration of Solvent Used With Oil-Soluble Preservatives 25 to 28 Penetration of Zinc-Containing Preservatives 29 to 32 Differentiating between Sapwood and Heartwood in Pine Species (Pinus sp.) 33 to 36 Differentiating between Sapwood and Heartwood in Douglas Fir (Pseudotsuga menziesii) 37 to 40 Differentiating between Sapwood and Heartwood in White Fir (Abies concolor) 41 to 44 Differentiating Between Woods of the Red Oak and the White Oak Species 45 to 48 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.