DIN EN ISO 11701-2010 植物的脂肪和油.使用光散射检测器用高效液相色谱法(HPLC)测定卵磷脂中的磷脂质含量
Vegetable fats and oils - Determination of phospholipids content in lecithins by HPLC using a light-scattering detector (ISO 11701:2009); German version EN ISO 11701:2009
This International Standard specifies a procedure for the quantitative determination of phospholipids by High Performance Liquid Chromatography (HPLC) using a Diol column and light scattering detection. The method is applicable to crude, oil containing lecithins and to oil-free lecithins and lecithin fractions from vegetable fats and oils. The method is not applicable to animal and ruminant lecithins and enzymatically hydrolyzed lecithins; the peak separation of lysophosphatidylethanolamine (LPE), lysophosphatidylinositol (LPI) and lysophosphatidic acid (LPA) is insufficient.