DIN EN ISO 12966-2-2011 动物和植物的脂肪和油.脂肪酸甲基酯的气相色谱分析.第2部分:脂肪酸甲酯的制备(ISO 12966-2-2011).德文版本EN ISO 12966-2-2011
Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 2: Preparation of methyl esters of fatty acids (ISO 12966-2:2011); German version EN ISO 12966-2:2011
This part of ISO 12966 specifies methods of preparing the methyl esters of fatty acids.
It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements four methylation methods are specified, namely:
a) a “rapid” transmethylation procedure under alkaline conditions;
b) a “general” transmethylation/methylation procedure under sequential alkaline and acid conditions;
c) a BF3 transmethylation procedure;
d) an alternative procedure using acid-catalysed transmethylation of glycerides.
Methyl esters so produced are used in various analytical procedures requiring such derivatives, e.g. gas-liquid chromatography (GLC), thin-layer chromatography (TLC), and infrared spectrometry (IR).