DIN EN ISO 13884-2005 动物和植物脂肪和油.用红外光谱测定法测定孤立的反式异构体
Animal and vegetable fats and oils - Determination of isolated trans isomers by infrared spectrometry (ISO 13884:2003);English version of DIN EN ISO 13884:2005
This International Standard specifies a method for the accurate determination of isolated trans bonds in
natural or processed long-chain fatty acids, fatty acid esters and triglycerides with trans isomer levels ≥ 5 %.
The method is not applicable, or is applicable only with specific precautions, to
fats and oils containing high levels (over 5 %) of conjugated unsaturation (e.g. tung oil),
materials containing functional groups which modify the intensity of the C-H deformation about the trans
double bond [e.g. castor oil containing ricinoleic acid or its geometrical isomer, ricinelaidic acid
(12-hydroxy-9-octadecenoic acid)],
mixed triglycerides having long- and short-chain moieties (such as diacetostearin), or in general,
any materials containing constituents which have functional groups which give rise to specific absorption
bands at or sufficiently close to interfere with the 966 cm-1 band of the C-H deformation of the isolated
trans double bond.
NOTE Dienes, such as cis-trans and trans-trans dienes, can affect the calibration.
For accurate determinations on materials with trans levels below 5 %, a standardized capillary GLC method is
recommended (for example, see ISO 15304).